IntegrateRNA provides GalNAc conjugated oligonucleotides containing a monovalent/trivalent GalNAc cluster attached to the 3'-end or 5'-end of the oligonucleotide. These conjugates are compatible with DNA, 2'-OMe, 2'-MOE, 2'F oligonucleotides including the options of phosphodiester or phosphorothioate linkages. To support clients' oligonucleotides therapeutic research, IntegrateRNA offers GalNAc-siRNAs, GalNAc-ASOs, GalNAc-antagomiRs and GalNAc-conjugated nucleic acid therapeutics. GalNAc conjugation is a highly effective method for delivering therapeutic oligonucleotides to specific liver tissues/cells.
GalNAc (N-Acetylgalactosamine) is a naturally occurring sugar that binds to the asialoglycoprotein receptor (ASGPR), which is highly expressed in the membrane of hepatocytes (liver cells). Coupling GalNAc sugars to stabilized oligonucleotide molecules allows them to target liver cells and carry out their function, being shuttled into the cytoplasm as a result of the GalNAc-ASGPR binding. Also, by decorating it to a certein delivery system as a targeting moiety, GalNAc has achieved compelling successes in the development of nucleic acid therapeutics in recent years. Furthermore, these GalNAc-oligonucleotides delivery systems have been used successfully in nonclinical species and in human to modulate the expression of many hepatocellular target RNAs.
Based on extensive experience in the lncRNA field, IntegrateRNA provides customers with a comprehensive lncRNA pull-down service, from the initial experimental design to the identification of the last interacting molecules. Our experienced scientists and advanced technologies enable us to provide consistent and high-quality data in a cost-effective manner.
Studies have shown that lncRNA has a conserved secondary structure, can interact with proteins, DNA and RNA, and participates in a variety of biological processes. With the development of high-throughput sequencing technology, more and more lncRNAs have been annotated, but the functions of most lncRNAs are still unclear. Therefore, the study of lncRNAs is a very broad unknown field and has great scientific value. RNA pull-down technology has played a vital role in the validation of lncRNA biology.
High-throughput screening for the interacting proteins of lncRNA is convenient by pull-down combined with LC-MS. IntegrateRNA utilizes LC-MS to identify captured protein complexes, and then screen specificity Interacting proteins with the target lncRNA.